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Description
Protein A Recombinant Rat mAb (S-2863)Product Specification Host Rat Antigen Protein A Clone Number S 2863 Antibody Type Rat mAb Isotype IgG2a Application ELISA Purification Protein G Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05% BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 20 C as supplied Dilution application dilution species ELISA 1: 5000 1: 10000 Background Protein A
Product Specification
| Host | Rat |
| Antigen | Protein A |
| Clone Number | S-2863 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a |
| Application | ELISA |
| Purification | Protein G |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| ELISA | 1:5000-1:10000 | / |
Background
Protein A is a 42 kDa cell-wall protein originally isolated from the non-pathogenic bacterium Staphylococcus aureus. Its defining feature is a string of five highly homologous immunoglobulin-binding domains that bind the Fc region of most mammalian IgG molecules with nanomolar affinity while leaving antigen-binding sites free. This exquisite specificity underpins its central role in biotechnology: Protein A is covalently immobilized on chromatography resins to create the gold-standard affinity medium for capturing monoclonal and polyclonal antibodies during downstream processing, enabling >99 % purity in a single step. Engineered variants, such as alkaline-tolerant or protease-resistant “Z-domain” forms, have further expanded its utility in large-scale biomanufacturing. Beyond purification, soluble Protein A conjugates and fusion proteins are employed in immunoprecipitation, biosensors, and targeted drug delivery, making it one of the most indispensable tools in modern life sciences.
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