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Description
Human RAB37 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against the Ras-related protein Rab-37 (RAB37). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Ras-related protein Rab-37 (RAB37) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Ras-related protein Rab-37 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | RAB37, also known as RAB37, is a member of the RAS oncogene family and is encoded by the RAB37 gene. Rab proteins are low-molecular-weight GTPases that are key regulators of vesicle trafficking. Reducing Rab37 levels by RNA interference results in impaired glucose-induced insulin secretion and a decrease in the number of granules tightly associated with the plasma membrane. It is a novel Rab GTPase specifically expressed in the MC-9 mast cell line and bone marrow mast cells. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.5 ★★★★★
Based on 1507 reviews
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Product Reviews
★★★★★ 5
Basic mouse that works - - for now.
Size: 1 Pack, Style: USB Receiver, Color: Blue
I ordered this mouse (in blue) to replace the recently deceased exact same model that I've used for the past 2 or 3 years. I like the simplicity of the M185 mouse and mostly the best bang for your buck! I caught this one on sale for less than a ten-spot, so I thought, "why not."
I'm hoping that this one will last a bit longer than my two previous M185 models, that averaged about 3 years each. This model seems plagued by a short life span and is predisposed to mysteriously and suddenly dying, without any obvious symptoms or warning of any kind - - you're working and clicking along, going about your day, when suddenly the cursor on the computer screen freezes and that's it. Nothing, zero, nada. Funny thing, I'm very careful not to abuse or drop the mouse, and keep a fresh battery installed and watch for leakage and all that stuff - - they just stop working, and I assume it is caused by some sort of inherent electronic fault in the circuitry of the previous M185's. Due to my loyalty to the brand and model (and since it was on sale) I decided to give it one more shot. They say that "third time's a charm".
I like the fact that the M185 is simple and uncluttered with extra buttons that I would never use for my purpose, which is simple web-browsing and the occasional text document production. The feel is solid, not heavy, but not cheap feeling, either. I'm used to the shape of this mouse, which to me is quite comfortable for long sessions, another reason why I kind of wanted to stay with this model. The buttons have an audible "click", which doesn't bother me at all, and the wheel moves smoothly and also has a clicking operation (which I rarely ever use). The overall surface of the mouse has a matte finish, but like my previous models I'm sure it will eventually wear shiny spots where my fingers reside and will attain a more "well-used" look in time. Connecting it was a breeze, too. I just plugged the dongle into a spare rear usb port of my desktop, and the mouse connected instantly, no problem. The dongle can be stored safely inside the battery compartment, so you can take the whole thing out of the house if needed for use elsewhere. The speed of 1000 dpi seems just right for my purposes. The one I ordered even came with its own AA Duracell battery, which I wasn't really expecting. This mouse also enters "sleep mode" when you don't use it for a while, and I can't say how many times I've got up and left it on all night. And that's not hard to do, as I'll explain next.
The only real negative to this mouse is the absence of any easily visible status indicator of it's on / off switch. The rear housing of this mouse is matte black, including the tiny switch where you turn it on and off with your fingernail, with only a very faint "on" lettering (also black), and very hard to see with my aging eyes. The laser is not lighted or visible when on, also adding to the confusion, especially in a darkened room. So, as a workaround, I took a tiny drop of green paint and placed it down inside the switch slider to visibly help indicate the "on" position, and likewise a dot of bright orange for the "off" position. Many of Logitech's more expensive models (and a few other manufacturers) include this color-coded switch indication. I guess for the $$, they had to cut corners somewhere - - a minor complaint.
In summary, this is a good basic mouse that works - - let's hope that this one will last longer than its two predecessors.
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Reviewed in the United States on February 17, 2023
★★★★★ 5
Dependable, Reliable
Size: 1 Pack, Style: USB Receiver, Color: Swift Grey, Size: 1 Pack, Style: USB Receiver, Color: Swift Grey
As advertised. Logitech, best mouse for your hard-earned cheddar!
It’s not that the (years) old Logitech mouse failed in any operational way, it just got that gummy, sticky surface texture that’s impossible to remove. While shopping for a replacement I noticed an off-brand mouse/keyboard combo and thought, “hey, 2 for 1”…mistake. All was well for about three weeks, long enough that when the gremlins arrived I didn’t immediately associate the new hardware. For the purposes of this discussion, gentle reader, know that I run legacy (what I affectionately refer to as, “Lazarus”) builds. Rigs that started life running Windows Vista/7, died, and that now, resurrected, run 10 and 11, and all that that implies. So, when a mouse started stuttering, of course I thought it was a gremlin in the machine, right? I spent more days than I am comfortable sharing with you pouring over processes and background apps, and registry trims…before Accam’s Razor suggested I eliminate the mouse as a variable. Changing the battery was ineffective, but when I swapped in the ooold Logitech…Yahtzee/Bingo/Booyah…smooth and precise…butter! All I needed was a new Logitech mouse.
So, if you’re here you likely are having mouse issues of your own, or you just need one that works…this is it!
😉
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Nice and a great value.
Size: 1 Pack, Style: USB Receiver, Color: Swift Grey
Hey, it works well. Just plug it in and you are ready to go. Only issue, minor, no light or color coding on back to indicate on or off.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2026
★★★★★ 5
Smooth and ergo nice mouse.
Size: 1 Pack, Style: USB Receiver, Color: Swift Grey
I needed this little thing quickly and got it within 2 hours of ordering. It was only an extra five bucks to get it now. Would have burned more than that in fuel. Works exactly as I would have expected. Perfect.
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Reviewed in the United States on June 11, 2026
★★★★★ 5
Simple and efficient
Size: 1 Pack, Style: USB Receiver, Color: Swift Grey
This is a dandy little item. Easy to use straight out of the box, No fancy bells and whistles.but why should a mouse try to replicate commands that are on the laptop?
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Reviewed in the United States on May 16, 2026