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Description
Human HSP90b1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Heat Shock Protein 90kDa Beta 1 (HSP90b1). After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Heat Shock Protein 90kDa Beta 1 (HSP90b1) in the sample. The absorbance (OD) is measured at 450nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Heat Shock Protein 90kDa Beta 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The 90 kDa heat shock protein β1 (HSP90β1) is a protein encoded by the HSP90B1 gene. This gene encodes a member of the adenosine triphosphate (ATP) metabolic chaperone family, which stabilizes and folds other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. A microRNA gene is located within the 5' exon of this gene. Pseudogenes of this gene are found on chromosomes 1 and 15. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.0 ★★★★★
Based on 2199 reviews
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Product Reviews
★★★★★ 5
Great moisturizer
Style: Deep Moisturizing, Size: 1.76 Fl Oz (Pack of 1)
This is a great product! The consistency is thick, but not heavy. No overwhelming fragrance. Applies easily and is absorbed quickly. Would definitely purchase again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 1, 2026
★★★★★ 5
NO REGRETS NO FRETS 🙂
Size: 2 Ounce (Pack of 1), Color: Tallow
Love it. Nothing bad. Soft, creamy, absorbs amazingly well, no odor, does wonders for back of hands from frequent hand washing, can fill a crack in skin with the tallow to heal a dryness related wound. Wash hands dry and the warm water and some soaps can strip skin oil and use this tallow to seal and/or replace moisture in your hands! No greasy issues. Truly a lifetime pick and my jar has easily another month usage and had it so far at least two months. Just a dab on back of palms and rub and work up on forearm and elbow. You will not be disappointed using it. I keep near the sink to remind me 🙂
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Reviewed in the United States on April 6, 2026
★★★★★ 5
Great product
Size: 2 Ounce (Pack of 1), Color: Tallow
Love this stuff, putting it on daily makes for very soft skin and it doesn't clog pores as I have been using it on my face without issue.. Its not like most animal made products it's not greasy it's actually very consistent and lasts all day
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Reviewed in the United States on May 28, 2026
★★★★★ 4
My new favorite moisturizer
Size: 2 Ounce (Pack of 1), Color: Tallow, Size: 2 Ounce (Pack of 1), Color: Tallow
This is the best moisturizer I’ve ever used on my hands. I struggle with dry hands in the winter from the cold and constant handwashing, so I’m always having to put moisturizer on to combat the dryness. My fiancé had started using beef tallow on his face (another more expensive brand) and was raving about it so I went searching on Amazon to get my own bottle. I chose this one based on the ingredients and the good reviews, as well as the price point. I love the way it melts into your hands leaving them silky smooth with no residue, greasiness, or stickiness. The reason that I deducted one star was because I felt the advertisement on Amazon was misleading. If you read the headline it makes it sound like the product has all organic ingredients. When I read the ingredient list once I received the product, the only ingredient that was listed as organic in the formula was the beeswax. I still love the product and I already bought another bottle, but I thought it was worth mentioning as I know that using 100% organic products is important to some people. I still highly recommend this product based on its effectiveness.
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Reviewed in the United States on February 7, 2026
★★★★★ 5
My skin feels nourished and radiant ✨
Size: 2 Ounce (Pack of 1), Color: Tallow
I’ve been using this balm for a little while now, and the difference is subtle but real. The texture is incredibly smooth and melts easily into my skin without feeling greasy. I can tell it’s made with really high-quality ingredients because it absorbs beautifully and leaves my skin feeling nourished immediately.
Since I started using it, the areas that usually feel dry—my hands and the sides of my face—are softer and more supple. Even the little cracks and rough patches have improved, and my skin looks and feels renewed and radiant. It’s not an overnight transformation, but it’s a steady, noticeable change that makes my skin feel healthy and cared for.
What I love most is how it strengthens my skin barrier. My skin feels more resilient and comfortable throughout the day, even in dry or cold weather. It’s like it quietly restores balance and keeps my skin happy without any heaviness or stickiness.
Using this balm has made my daily skincare feel a little more nurturing and confident—my skin looks smoother, softer, and truly glowing 🐝💛
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Reviewed in the United States on March 23, 2026
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