Recombinant Carboxypeptidase B,Bovine
SKU: 30258998695

Recombinant Carboxypeptidase B,Bovine

Sale price$131.40 Regular price$146.00
Save 10%

Shipping Estimate
USA
  • USA
  • CAN

Ships within 48 hours · Estimated delivery Jul 7 - Jul 12

Promo Codes Available:

For Your Every Summer RSVP, with Code: SUMMER15

Description

Recombinant Carboxypeptidase B,BovineProduct Specification Species Bovine Synonyms Carboxypeptidase B Expression System PichiaPastoris Molecular Weight 33 kD Purity 95% by SDS PAGE & HPLC Tag His Tag Physical Appearance Lyophilized Powder Storage Buffer 20mM Tris HCl 100mM NaCl Reconstitution Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris HCl (pH7. 6) to make the concentration of enzyme solution 1 10mg ml. Stability & Storage 24 month, 2 to 8 C under

Product Specification


Species Bovine
Synonyms Carboxypeptidase B
Expression System PichiaPastoris
Molecular Weight

33 kD

Purity >95% by SDS-PAGE & HPLC
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer 20mM Tris-HCl 、100mM NaCl
Reconstitution

Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris-HCl (pH7.6) to make the concentration of enzyme solution 1-10mg /ml.

Stability & Storage

·24 month, 2 to 8 °C under sterile conditions
. After dissolution, store at -20℃ and avoid repeated freeze-thaw cycles.

Reference

[1] Akparov V, Sokolenko N, Timofeev V, Kuranova I. Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine. Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1335-40.
[2] James W. Brodrick, Michael C. Geokas, Corey Largman, Human carboxypeptidase B. II. Purification of the enzyme from pancreatic tissue and comparison with the enzymes present in pancreatic secretion, Biochimica et Biophysica Acta (BBA) - Enzymology, 1976, 468-481.

Background

Carboxypeptidase B, also known as peptidyl-L-lysine (L-arginine) hydrolase, contains one Zn atom per molecule and can selectively hydrolyze arginine and lysine at the carboxyl end of proteins or polypeptides. CPB can be used in the production of recombinant insulin and its analogues, and the quality detection of recombinant antibodies. and also used in the production of other recombinant polypeptides.

Components

20mM Tris-HCl 、100mM NaCl、Mannitol、Carboxypeptidase B

Protocol

The recommended amount of Carboxypeptidase B is 1:50~1:1000 (w/w) compared with the target protein (mass ratio), the optimal pH is 7.5-9.0, and the appropriate enzyme digestion temperature is 25℃~37℃.

Guidelines

1. For different protein samples, it is necessary to explore the optimal enzyme concentration and reaction time.

2. Reaction at 25℃~37℃, the reaction time can be appropriately extended when digestion at 25℃.

Unit Definition

The amount of enzyme that catalyzes the hydrolysis of 1 μmol of maroyl-L-arginine in 1 min is one enzyme activity unit of carboxypeptidase B.
Shipping Notes
  • Free Standard Shipping on $100+ Orders to the USA.
  • Except Preorder products are shipped in 48 hours.
  • Delivery to the USA:
  1. Standard Shipping : 3-10 business days
  • If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 30258998695

Discover Niche Categories That Outsell

Top-Converting Item to Boost Your Average Order

4.7 ★★★★★
Based on 1467 reviews
Sort
Highest Rating
Newest First
Oldest First
Product Reviews
B
Verified Purchase
Beth in Texas
Lexington, US
★★★★★ 5
Great blouse for work!
I purchased this red blouse as a Prime deal for Valentine’s Day. I wore to work with black pants. I think this is a great work blouse and intend to buy it in more colors. It is cute, not very formfitting, but still has a V-neck. I would say it runs slightly large. It is also long in length. For reference, I am usually a large or XL because I am busty, but the large was plenty big and I was wondering if I could fit into a medium... If you want a more flowing top, this is definitely a great choice. I think this would look great with white jeans also and perhaps even tied at the waist. As some reviewers have said, it does wrinkle easily. wWhen I got it from Amazon, I ironed it inside out, and all of the wrinkles came out. Perhaps, if you were sitting all day, it would be very wrinkly in the front. I sit for my job, but I did not tuck in the blouse - I kind of had it flow away from my body and it stayed mostly wrinkle free all day. I plan to buty in more colors!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 12, 2023
D
Verified Purchase
diane trowbridge
New York, US
★★★★★ 3
Lovely top
Size: Large, Color: Mini Leopard
Beautiful top and size was true to what was listed.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 12, 2026
P
Verified Purchase
Poopylou
Omaha, US
★★★★★ 5
Very nice
Size: Large, Color: Blue White Petal
Perfect length and fit and doesn't cling if you like a more relaxed look. I purchased 3 of these for work and I love them. I can wear them with khaki cropped pants and sandals, very cute. After washing and line drying there hasn't been any shrinkage and the fabric stays nice. Perfect for warmer weather climates!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 18, 2026
R
Verified Purchase
R daugh
Cuba, US
★★★★★ 5
Relaxed fit, business or casual summer blouse.
Size: X-Large, Color: Mini Leopard
Light weight summer material, soft, nice feel. Fits well. Color as advertised. I recommend highly.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 24, 2026
M
Verified Purchase
Maris Goldberg
Battle Creek, US
★★★★★ 4
Sizing seems to be different depending on color!
Size: Large, Color: Mini Leopard
Ordered in Mini Leopard in Large and Pink in Medium. The Mini leopard was too big and the Pink was to small! Decided to return both and order the pink in large. That fits!!! Sizing is different in different colors! Like the style and hopefully it will wash okay. Kept the pink, large! It did come extremely creased!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 27, 2026

recommand products